Yosetime Sam is one of the antagonists of the Looney Tunes franchise. In Looney Tunes Dash!, Yosemite Sam is the main antagonist of the Daffy levels alongside Temple Pig, minor antagonist in the Tweety levels, tertiary antagonist of the Bugs Bunny levels and secondary antagonist of the Road Runner levels. In the Daffy levels, he is defeated when Daffy locks himself in a treasure chest and kicked foot on on it.
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